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. Author manuscript; available in PMC: 2013 Mar 19.
Published in final edited form as: J Neurosci. 2012 Sep 19;32(38):13206–13220. doi: 10.1523/JNEUROSCI.5041-11.2012

Figure 3. In vitro characterization of tet-off-NT-3 virus-transduced cells.

Figure 3

(A–D) GFP fluorescence is easily detected 48 h after transfection of 293T cells with (A) 40 ng and (B) 20 ng virus without doxycycline (−Dox) treatment, whereas only occasional GFP positive cells are present in (C, D) wells treated with doxycycline (+Dox, 1 μg/ml). (E) ELISA indicates that NT-3 levels in supernatants of cells without doxycycline are significantly higher than NT-3 levels in supernatants from cells treated with doxycycline. Scale bar: 200 μm (***p < 0.001; ANOVA followed by Fisher’s post hoc test).