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. Author manuscript; available in PMC: 2014 Jan 15.
Published in final edited form as: J Neuroimmunol. 2012 Oct 26;254(1-2):110–116. doi: 10.1016/j.jneuroim.2012.10.005

Figure 5. T cell cytokine production.

Figure 5

WT and EBI3−/− mice were injected i.p. with JHMV and splenocytes isolated at day 7 p.i. to evaluate expression of IFN-γ and IL-10 in response to viral peptide stimulation. (A) Intracellular IFN-γ staining reveals diminished frequency of M133–147-specific CD4+ T cells in EBI3−/− mice compared to WT whereas there were similar frequencies of virus-specific CD8+ T cells. Splenocytes from infected WT and EBI3−/− mice were stimulated with either 5 µM M133–147 or S510–518 peptides for 48 hours and secretion of IFN-γ (B) or IL-10 (C) determined by ELISA. * indicates p≤0.05 and ** indicates p≤0.01.