Skip to main content
. Author manuscript; available in PMC: 2013 Jul 19.
Published in final edited form as: J Vis Exp. 2013 May 19;(75):10.3791/50239. doi: 10.3791/50239

Figure 2. Representative cell cycle analysis of tissues expressing GFP using Vybrant DyeCycle Violet and the Attune.

Figure 2

A representative workspace, containing 4 dot plots and 2 histograms is shown for a larval wing sample expressing GFP and Cyclin D in the posterior wing, driven by an engrailed-Gal4 transgene. A. Dot plot of cell size with Gate 1 to exclude debris and clumps. B. Dot plot to discriminate singlets with Gate 2 to exclude unstained cells, sub-G1 DNA content (apoptosis), and clumps. C. Dot plot of GFP vs. DNA content. GFP negative (Gate 3) and positive (Gate 4) cells can be distinguished and 2N and 4N DNA content is evident based upon position along x-axis. D. Dot plot of cell size as measured by GFP vs. forward scatter (FSC) to generate Gates 5 and 6. Note that cells within Gate1 are colored red for visual assistance in determining Gates 5 and 6 for this scatter plot. E. Histogram of DNA content vs. counts for GFP or RFP negative (population set to Gate 3). F. Histogram of DNA content vs. counts for GFP or RFP positive (population set to Gate 4). Histograms can also be included for cell size if desired. For cell size histograms, populations should be set to Gates 5 and 6.