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. Author manuscript; available in PMC: 2012 Dec 20.
Published in final edited form as: J Neurosci. 2012 Jun 20;32(25):8725–8731. doi: 10.1523/JNEUROSCI.1160-12.2012

Figure 2.

Figure 2

Wnt7A is expressed in embryonic γ-MNs. A–A″, Wnt7a is expressed in the ventricular zone (arrow) and is absent from the ventral motor neuron region in E15.5 Hb9::GFP mice (A, A′). A″, Magnified boxed region in A. B–B″, At E16.5, Hb9::GFP LOW/Wnt7aON cells are interspersed with Hb9::GFP ON/Wnt7aOFF cells in the ventral spinal cord. Wnt7a labeling is also present in the ventricular zone (B, B′). B′, High magnification of boxed region in B. C, C′, E, F, Wnt7aON cells are localized throughout the motor neuron nucleus of E17.5 Hb9::GFP mice and are segregated from Hb9::GFP ON cells. E, Split and merged channels of C′. D, D′, Wnt7aON cells are absent from the ventral spinal cord of E17.5 Gfrα1 −/−; Hb9::GFP mice (D), while Hb9::GFP ON/Wnt7aOFF cells persist (D′). G, H′, Wnt7aON cells in the motor nucleus of E17.5 control mice (G). RetCreERT2/−mice lack Wnt7aON cells at E17.5 (H ). ChATON cells in control (G′) and RetCreERT2/−mice (H′). Split and merged channels shown for A″, B″, E, and F. Scale bars: A–H′, 100 μm.