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. Author manuscript; available in PMC: 2012 Dec 20.
Published in final edited form as: J Neurosci. 2012 Jun 20;32(25):8725–8731. doi: 10.1523/JNEUROSCI.1160-12.2012

Figure 3.

Figure 3

A–C, A spindle-derived GDNF-independent signal is required for γ-MN development. Wnt7aON putative γ-MNs are localized within the motor neuron nucleus of P0 (A), P4 (B), and P15 (C) control mice. D–F, Wnt7a labeling is eliminated in P0 (D), P4 (E), and P15 (F ) Pva::Cre; Isl2::DTA mutant mice. G–I, Wnt7aON γ-MNs are localized within the ventral spinal cord of P0 (G) and P5 (H ) Egr3−/−mice, but are lost at P15 (I ). J–J−, In control mice, NeuN OFF/ChATON γ-MNs (arrows in J′) intermingle with NeuN ON/ChATON α-MNs at P4. K–K″, NeuN OFF/ChATON γ-MNs are absent in P4 Pva::Cre; Isl2::DTA mice. L–L″, In P0 wild type mice, Wnt7aON putative γ-MNs (arrow) are localized within the Cm motor pool, identified by coexpression of Islet1 and Pea3. Scale bar: A–L″, 100 μm.