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. Author manuscript; available in PMC: 2013 Apr 17.
Published in final edited form as: J Neurosci. 2012 Oct 17;32(42):14465–14477. doi: 10.1523/JNEUROSCI.2246-12.2012

FIG. 4. Nox1 knockdown inhibits aggregation of overexpressed WT α-synuclein in N27 cells induced by PQ.

FIG. 4

(A) Representative pictures of flag tagged WT α-synuclein immunoreactivity (red) in control and PQ treated cells. Upper panels scale bars = 50 µm and lower panels scale bars = 10 µm. (B) flag tagged WT α-synuclein fluorescence immunostaining of N27 cells incubated with Nox1 shRNA/LVX (shNox1-ZsGreen) viral particles and exposed to 800 µM PQ. shNox1-ZsGreen infected cells were identified by green fluorescence (ZsGreen) in cells. Scale bars = 10 µm. (C) Quantification of the bright, punctuated fluorescent cells, indicated with arrowheads in (A) and (B). More than 20 assigned fields were analysed in each independent experiment and in average the minimum number of total cells counted per condition was 400 cells. Data are shown as the mean ± SEM. Statistical analysis was performed using one-way ANOVA or two-way ANOVA, followed by Bonferroni’s Multiple Comparison Test. **P<0.01 and ***P<0.001. Arrowheads specify cells with aggregated α-synuclein pattern, and arrow indicates N27 cells depicting double-staining for shNox1-ZsGreen and α-synuclein-flag. Ctr: control and PQ: paraquat.