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. Author manuscript; available in PMC: 2013 Apr 17.
Published in final edited form as: J Neurosci. 2012 Oct 17;32(42):14532–14537. doi: 10.1523/JNEUROSCI.3509-12.2012

Figure 4. β-alanine -sensitive neurons are C-mechano-heat nociceptive (CMH) neurons and do not respond to histamine.

Figure 4

(A) Bright-field image of a neuronal recording (arrow) with an extracellular electrode (outlined with dashed blue lines). (B) Fluorescent microscopy revealed the expression of GFP (i.e., MrgprD+) in this neuron. (C) Location of the cutaneous receptive field (RF, red dot) of this neuron on the hairy skin of the hind-paw and conduction velocity (0.64 m/s, lower trace) obtained with electrical stimulation (arrow) of the RF. (D) Responses of this neuron to a 60 mN force via a 200 μm diameter probe applied to the RF (1 sec) with original extracellular recording trace and action potentials (AP) indicated below. (E) Response to heat stimulation (38 to 51°C, 5 sec) indicates that this is a CMH nociceptive neuron. (F) Responses of the above CMH neuron to the intradermal injection of vehicle (VEH), histamine (HIS) and β-alanine (BAL).