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. Author manuscript; available in PMC: 2013 May 7.
Published in final edited form as: J Neurosci. 2012 Nov 7;32(45):16018–16030. doi: 10.1523/JNEUROSCI.1685-12.2012

Figure 6. Dnlg2 is required pre- and post-synaptically for proper synaptic growth at NMJs.

Figure 6

(A–J) dnlg2 cDNA transgene rescue analyses at NMJ 6/7. Compared to two dnlg2 mutants, dnlg2CL2 (B) and dnlg2KO70 (C, Sun et al., 2011), expression of Dnlg2 in muscles with C57-Gal4 (D, E) or 24B-Gal4 (F, G) failed to rescue bouton number deficits in both dnlg2CL2 and dnlg2KO70 mutants. Similarly, expression of Dnlg2 in neurons using elav-Gal4 (H) also failed to rescue the NMJ phenotype. However, when Dnlg2 was expressed ubiquitously with tub-Gal4 (J, I), the NMJ phenotype in both dnlg2CL2 and dnlg2KO70 mutants was restored to wild type levels.

(K–N) dnlg2 overexpression analyses in the wild type background. Overexpression of Dnlg2 in muscles using C57-Gal4 (K) or 24B-Gal4 (L) adversely affected bouton growth. In contrast, overexpression of Dnlg2 in neurons (M) does not affect NMJ bouton growth or number dramatically. Simultaneous overexpression of Dnlg2 in muscles and neurons promotes bouton growth at NMJs (N).

(O, P) Quantification of bouton number at NMJ6/7 for rescue analyses (O) and overexpression analyses (P). Error bar = SEM; n.s.: not significant; ***p < 0.001; **p < 0.01; *p < 0.05 (ANOVA, Tukey post hoc test). Scale bar= 20μm.