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. Author manuscript; available in PMC: 2013 May 7.
Published in final edited form as: J Neurosci. 2012 Nov 7;32(45):15934–15945. doi: 10.1523/JNEUROSCI.3227-12.2012

Fig. 5. Engraftment of endogenous CP by primary and derived CPECs following intraventricular injection.

Fig. 5

(A–C) Primary CP cell injections, single or collapsed Z-stack images of ipsilateral telencephalic choroid plexus two days after injection. Injected H2B-GFP (A) or CFDA-SE-labelled CP cells (B) preferentially associate with host CP, which expresses Aqp1 (red). Confocal projections reveal labelled cells on the nuclear side of the continuous Aqp1-positive apical border (arrowheads in C), indicating integration of injected cells into host CP with appropriate apicobasal polarity. (D–F) Mouse ESC-derived (M1) aggregates, no BMP4 treatment, two days after injection. Dye-labelled cells are associated with ipsilateral host CP, but remain on the ventricular side of the Aqp1-expressing apical border (arrows in F), indicating a failure to engraft host CP. (G–I) Mouse ESC-derived (M1) aggregates, with BMP4 treatment and Ara-C enrichment, 1–2 days after injection. Like the primary cells, most of the dye-labelled cells associate with host CP and are appropriately polarized on the nuclear side of the endogenous Aqp1 border, indicating host CP engraftment. (J–K) Quantification of distributions and integration of injected BMP4-treated and -untreated cells. Cells from both groups are found mostly in association with CP (J), but the BMP4-treated cells integrate into host CP with much greater efficiency (K), consistent with their dCPEC identity (see Results for numbers). Scale bars: 50 um.