Diffusion tensor imaging (DTI) provides rich information on white matter structures, connectivity patterns, as well as the integrity of these connections in the mouse brain. Among the parameters calculated from DTI are: FA (fractional anisotropy), AD (axial diffusivity), RD (radial diffusivity), and Clr (color-coded orientation map of the primary eigenvector. Green: left-to-right; red: rostral-to-caudal; blue: dorsal-to-ventral). The right column shows magnified views that correspond to the regions specified by the rectangles (a, b, and c) in the left column. Arrows indicate structures that are better discerned using directional information on the colormap: 1. Habenular commissure; 2. Fimbria; 3. Optic tract; 4. Cerebral peduncle; 5. Thin branch of optic tract adjacent to 6. Thin branch of fimbria; 7. Cingulum (rostro-caudal orientation); 8 Corpus callosum (medial to lateral orientation). Reproduced with permission from (Jiang and Johnson 2010).