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. Author manuscript; available in PMC: 2013 May 14.
Published in final edited form as: J Neurosci. 2012 Nov 14;32(46):16314–16330. doi: 10.1523/JNEUROSCI.2097-12.2012

Figure 10. Cultured spiral ganglion neurons express multiple Ca2+ channels.

Figure 10

A Cultured (48 hrs in culture) apical spiral ganglion neurons were fixed and labeled with antibodies against Cav1.3, Cav2.1, Cav2.2, Cav2.3, Cav3.1, Cav3.2, and Cav3.3. As shown, neurons were labeled with the neuronal marker Tuj1 (in red, left panels, a1–a7), the channels (in green, middle left panel), and the nuclei were stained with DAPI (in blue) and the merged images (right panel). Spiral ganglion neurons stained positively for Cav1.3, Cav2.1, Cav2.2, Cav2.3, Cav3.1, Cav3.2, and Cav3.3. B Similar data were obtained for basal neurons as shown in b1–b7 for Tuj1, nuclei stain and channels Cav1.3, Cav2.1, Cav2.2, Cav2.3, Cav3.1, Cav3.2, and Cav3.3, respectively. Scale bar = 10 μm.