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. Author manuscript; available in PMC: 2013 May 14.
Published in final edited form as: J Neurosci. 2012 Nov 14;32(46):16510–16520. doi: 10.1523/JNEUROSCI.2631-12.2012

Figure 6.

Figure 6

Increased association between PKCζ/Ι and ZIP/p62 in ventral spinal segments containing phrenic motor neurons. A. Representative images depicting CTB (green), aPKC (red) and ZIP/p62 (blue) in the C4 ventral horn. Merged images on right demonstrate co-localization of ZIP/p62 and aPKC in CTB+ phrenic motor neurons. The phrenic motor nucleus is encircled in top panels. Scale bar on top panel = 200 μm, bottom panel = 20 μm. B, C. PKCζ/Ι was immunoprecipitated from ventral C4 gray matter homogenates; immunoprecipitates were then immunoblotted for ZIP/p62. B. Representative immunoblots of ZIP/p62 10 or 60 following resumption of respiratory neural activity after a neural apnea, or equivalent time points in time controls. C. Average change in ZIP/p62 density from time controls illustrating significant increases in ZIP/p62 binding to PKCζ/Ι 10, but not 20 or 60 min after resumption of respiratory neural activity. *significantly different from zero, p<0.05