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. Author manuscript; available in PMC: 2013 Jul 1.
Published in final edited form as: Protein Expr Purif. 2012 Nov 29;90(1):40–46. doi: 10.1016/j.pep.2012.11.005

Table 1.

Data collection and processing parameter

Native PlcHR2 Se-Met PlcHR2
Beam line X10SA X06SA
Wavelength [Å] 1.000 0.95370
Temperature [K] 100 K 100 K
Oscillation range [°] 1 1
No. of frames 180 170
Unit cell dimensions [Å], [°] 175.5, 196.4, 325.3 157.9, 75.4, 141.0 β = 93.2
Space group C2221 C2
max. resolution [Å] 4.0 2.75
No. observed reflections 150 089 150 083
No unique reflections 45 739 43 080
Completeness (last shell) 95.6 (92.2) 99.7 (99.3)
R sym* (last shell) 0.103 (0.352) 0.130 (0.308)
I/σ (last shell) 8.3 (2.7) 9.3 (4.7)
*

R = SUM (I-<I>)2/SUM(I2)