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Characterization of the nano-precipitation results at various protein concentrations at a 1:4 volume ratio of water-to-acetonitrile.
| Protein | Concentration (mg/ml) | Precipitation yield (%) | Insoluble aggregates (%) | Residual activity (%) | Diameter (nm) |
|---|---|---|---|---|---|
| Lysozyme | 10 | 70 ± 11 | 2 ± 1 | 87 ± 5 | 86 ± 16 |
| 20 | 95 ± 4 | 1 ± 0 | 83 ± 1 | 92 ± 15 | |
| 30 | 99 ± 1 | 1 ± 1 | 96 ± 2 | 168 ± 14 | |
| α-Chymotrypsin | 10 | 77 ± 5 | 0 ± 0 | 78 ± 0 | 66 ± 16 |
| 20 | 83 ± 3 | 0 ± 0 | 100 ± 1 | 174 ± 16 | |
| 30 | 83 ± 4 | 0 ± 0 | 100 ± 2 | 200 ± 12 |
