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Effect of the ratio of dipersing-to-diffusing phase on the encapsulation efficiency of lysozyme in PLGA nanoparticles.*
| Ratio of dispersing phase to diffusing phase |
Encapsulation efficiency (%) |
|---|---|
| 1:10 | 9 ± 3 |
| 1:20 | 49 ± 9 |
| 1:30 | 71 ± 7 |
| 1:40 | 84 ± 8 |
Protein concentration: 25mg/ml; volume ratio between water and ACN: 1:4; PLGA 50:50 concentration in ACN: 90mg/ml; theoretical loading: 5% (w/w).
