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. Author manuscript; available in PMC: 2013 Feb 7.
Published in final edited form as: J Immunol. 2012 Dec 26;190(3):1048ā€“1055. doi: 10.4049/jimmunol.1202455

Figure 5. Adoptive transfer of wild-type IM into DT-treated chimeric mice restores the PPS14- and PC-specific IgM and IgG responses.

Figure 5

(A) Spleen cells from WT FVB mice were enriched for CD11b+ cells using magnetic cell sorting (left panel) followed by purification of CD11b+Ly6Chi cells by electronic cell sorting (right panel) [>99% purity]. (B) Purified CD11b+Ly6Chi cells (2 Ɨ 105 cells/mouse) were injected i.v. into CD11b-DTR BM chimeric mice 24 h after DT injection. DT was administered every 2ā€“3 d for the duration of the experiment. DT-treated chimeric mice without injected CD11b+Ly6Chi cells and chimeric mice without injection of DT or CD11b+Ly6Chi cells were included as controls. All groups consisted of 7 mice each. Serum titers of antigen-specific IgM and IgG were measured by ELISA. *Significance pā‰¤0.05. One of two representative experiments.