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. Author manuscript; available in PMC: 2013 Feb 7.
Published in final edited form as: J Immunol. 2012 Dec 26;190(3):1048–1055. doi: 10.4049/jimmunol.1202455

Figure 7. IM are phenotypically and functionally distinct from circulating CD11b+CD11clowLy6G−/C− cells (immature “blood DC”).

Figure 7

(A) Expression of CD11c (PE-anti-CD11c) on gated CD19-CD90-Ly6G- cells (Alexa Fluor 700-anti-CD19, anti-CD90, and anti-Ly6G) from naïve WT mice followed by Ly6C (FITC-anti-Ly6C) and CD11b (BV421-anti-CD11b) expression on gated CD11cneg, CD11clow, and CD11chigh cells. (B) WT mice were injected with Alexa Flour 405-labeled R614 (red). 4 h post-injection, spleens were obtained and sections were stained with FITC-anti-Ly6C (green) + PE-anti-CD11b (blue).