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. Author manuscript; available in PMC: 2013 Jan 29.
Published in final edited form as: Biochem J. 2012 Aug 1;445(3):377–382. doi: 10.1042/BJ20120820

Figure 3. IL-1 pretreatment accelerates down-regulation of CYP2B by the NO donor NOC-18.

Figure 3

(A) Three day-old hepatocytes were pretreated with 1 mM PB, which was present for the rest of the experiment. 48 h later, the cells were treated with L-NAME (100 µM) with or without IL-1 (5 ng/ml) for a period of 12 h. Subsequently, NOC-18 (500 µM) was added directly to the media, and cells were harvested 6 or 12 h after NOC-18 addition. (B) Western blot of CYP2B in cell lysates. (C) Quantitative analysis of the data in panel B. Values are means ± SEM normalized to the GAPDH signals, and are expressed relative to the 6 h L-NAME control group. The figures within the bars of the NOC-18-treated groups indicate the percentage decreases relative to the respective controls. a, P<0.05 compared to control at same time point; b, P<0.05 compared to NOC-18-treated samples without IL-1 pretreatment. One-way ANOVA and Tukey’s test.