Figure 2. Correlation between spatially matched apparent diffusion coefficient measurements and cell density from stereotactic biopsy samples.

(A) Postoperative, high-resolution 3D T₁-weighted anatomical MRI showing the biopsy location (arrow) in single patients. (B) Representative histological images (hematoxylin and eosin, ×20 magnification) showing how cell density increases with an increase in tumor grade (scale bars: 50 μm). (C) Spatially matched ADC measurements taken from the biopsy location in the same four patients as in (B), showing a decrease in ADC with an increase in tumor grade and cell density. (D) Scatter plot of average ADC within stereotactic biopsy locations and average cellularity for 17 patients (circles) shows a significant linear correlation (Pearson's correlation coefficient, r² = 0.7933; p < 0.0001) between mean ADC and mean cell density in nuclei/mm². (E) Correlation between mean ADC and mean cell density in nuclei/HPF. (D & E) Dashed black line indicates the linear regression while the dashed gray lines indicate the 95% CIs for the regression.

ADC: Apparent diffusion coefficient; HPF: High power field. Reproduced with permission from John Wiley and Sons (License No. 2901470746078) [22].