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. Author manuscript; available in PMC: 2014 Jun 1.
Published in final edited form as: Mol Oncol. 2013 Jan 19;7(3):555–566. doi: 10.1016/j.molonc.2013.01.003

Figure 2.

Figure 2

siRNA was used to deplete endogenous LSD1 in cultured hormone dependent (LnCaP: panels A,D,H,N,Q) and hormone independent cells (LnCaP:C4-2, panels B,E,I,L,O,R and PC3, panels C,F,J,M,P,S). siRNA effectively reduced LSD1 protein (A–C) and mRNA (D–F) as compared to the cells transfected with an siRNA targeting luciferase. Reduced LSD1 levels decreased VEGF-A mRNA (H–J). Functional depletion of LSD1 reduced PSA/KLK3 mRNA expression in hormone dependent LnCaP cells (K), but had no effect in hormone independent prostate cancer cells (L,M). Functional depletion of LSD1 reduced Tmprss2 mRNA expression in LnCaP and PC3 cells (N,P). Secreted VEGF-A protein levels were quantified using an ELISA and compared to cultured prostate cancer cells transfected with luciferase control siRNA. Knockdown of LSD1 decreased secreted VEGF-A levels in hormone dependent LnCaP (Q) and hormone independent LnCaP:C4-2 and PC3 (R,S) cells P values: *=p<0.05, **=p<0.01, ***=p<0.005. An uncropped western is present as supplemental Figure 4.