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. Author manuscript; available in PMC: 2014 Jun 1.
Published in final edited form as: Mol Oncol. 2013 Jan 19;7(3):555–566. doi: 10.1016/j.molonc.2013.01.003

Figure 3.

Figure 3

We used the integrated genome viewer to assess LSD1 recruitment to the VEGFA and CCNA1 loci in the publically available ENCODE LSD1 ChIPseq dataset (GSM831002). Consistent with our clinical data indicating a functional relationship between LSD1 and VEGFA expression, LSD1 is shown to localize to the VEGF-A locus (A) but not cyclinA1 locus (B). siRNA depletion of LSD1 impairs androgen induced transcription of VEGF, PSA, Tmprss2. Androgen responsive LnCaP cells were transfected with siRNA targeting LSD1 and lucisferase (luc) as control. Transfected cells were treated with the synthetic androgen (R1881) and the effects on transcription of androgen regulated genes relative to ethanol vehicle controls were examined. Depletion of LSD1 (C) blocked androgen induced transcription of (D) VEGF-A, (E) PSA and (F) Tmprss2, indicating that LSD1 is required for the AR-mediated transcriptional activation of these loci. P values: *=p<0.05, **=p<0.01, ***=p<0.005, ****=p<0.0001.