Table 1.
Expression levels of top 50 enzyme genes in plasma membrane and cytosol with cathepsins in pancreatic cancer cell line, capan-2 and AsPC-1, with Affymetrix GeneChip Human Genome U133 Plus 2.0.
| Enzyme | Capan-2 | AsPC-1 |
|---|---|---|
| Cathepsin B | 1526.6 | 38 |
| Zinc Metalloproteinase | 1057.9 | 48 |
| Cathepsin H | 913.4 | 58.6 |
| Cathepsin D | 891.2 | 3.7 |
| gamma-Glutamyl Hydrolase | 840.6 | 20.3 |
| Calpastatin (CAST) | 746.2 | 28.3 |
| Aminopeptidase B | 726.2 | 14.9 |
| Leucine Aminopeptidase | 700.7 | 15.1 |
| Cathepsin C | 593.4 | 17.6 |
| Puromycin Sensitive Aminopeptidase | 530.2 | 4.9 |
| Prolylcarboxypeptidase | 516.3 | 16.8 |
| Aminoacylase 1 | 511.9 | 16.2 |
| Cytochrome P450 | 432.4 | 13.5 |
| N-Acylaminoacyl-Peptide Hydrolase | 335.5 | 9.7 |
| Tripeptidyl peptidase II (TPP2) | 296.7 | 36.4 |
| Cathepsin U | 288.4 | 7.4 |
| Aminopeptidase P | 264 | 328.2 |
| Dipeptidylpeptidase III (DPP3) | 257.5 | 4.2 |
| Leucine Aminopeptidase | 238 | 20.7 |
| Mitochondrial Intermediate Peptidase (MIPEP) | 206.8 | 35 |
| Aminopeptidase PILS (APPILS) | 195.1 | 2.4 |
| UDP-N-Acetylglucosamine-2-Epimerase (GNE) | 180.4 | 42.3 |
| Caspase 6 | 179 | 0.8 |
| Sentrin-Specific Protease (SENP2) | 176 | 116.5 |
| Glycosylasparaginase | 176 | 130.1 |
| Carboxypeptidase D | 168.2 | 57.3 |
| Transmembrane Protease(TMPRSS4) | 167.8 | 3.9 |
| Cysteine Protease | 167.1 | 17.1 |
| Aspartyl Aminopeptidase (DNPEP) | 163.6 | 24.7 |
| Peptidase D (PEPD) | 163.2 | 3.3 |
| Heparan Sulfate (glucosamine) 3-O-Sulfotransferase 1 (HS3ST1) | 157.1 | 5.3 |
| Aspartylglucosaminidase | 121.5 | 28.6 |
| GPI Transamidase | 119.6 | 112.6 |
| SentrinSUMO-specific protease 3 (SENP3) | 117.8 | 111.9 |
| Caspase 3 | 114.8 | 5.1 |
| Carboxy-Terminal Hydrolase | 111.7 | 56 |
| Caspase 8 | 108.8 | 49.8 |
| Phosphatidylcholine 2-acylhydrolase (cPLA2) | 108.3 | 3.3 |
| Polypeptide 5 (RPS6KA5) | 103.3 | 32.1 |
| beta-Site APP-Cleaving Enzyme | 100.2 | 178.3 |
| Protease 5 (isopeptidase T) (USP5) | 99.9 | 84 |
| Arginyl Aminopeptidase (Aminopeptidase B)-Like 1 (RNPEPL1) | 99.3 | 16.1 |
| SentrinSUMO-Specific Protease (SENP1) | 98.8 | 48.3 |
| Carboxypeptidase M | 97.4 | 7.8 |
| Carboxypeptidase D | 96.9 | 4.9 |
| 3-Phosphoinositide Dependent Protein Kinase-1 (PDPK1) | 94.7 | 53.3 |
| Putative Metalloglycoprotease | 94 | 55.4 |
| Aspartylglucosaminidase (AGA) | 93.2 | 12.5 |
| Carboxypeptidase 1 | 92.8 | 15 |
| Matrix Metalloproteinase 14 (MMP14) | 88.5 | 10.3 |
BOLD: selected for enzyme inhibition studies.