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. Author manuscript; available in PMC: 2013 Jul 23.
Published in final edited form as: J Neurosci. 2013 Jan 23;33(4):1577–1588. doi: 10.1523/JNEUROSCI.1749-12.2013

Figure 6.

Figure 6

GluA1 overexpression increases synaptic properties of GluA2-lacking AMPA receptors in CeA neurons. A, Illustration of stimulation (S) placement in BLA, and recording (R) of a viral vector-transfected neuron and EPSC in a CeA slice. A transfected cell was identified by the GFP fluorescence (top middle) and recorded in whole-cell configuration (top right) with a typical postsynaptic current (PSC) and excitatory postsynaptic current (EPSC) in the presence of picrotoxin (50 μM) (bottom right). B, Representative AMPA EPSCs and NMDA EPSCs in a control cell and in transfected cells from animals after single-side CeA injection of the mixture (GluA1-GFP) of AAV-GluA1 and AAV-GFP vectors (3:1) or AAV-GluA1-shRNA-GFP vector. C, Summarized results of the AMPA/NMDA EPSC ratio in the three indicated cell groups. Numbers in columns are cell numbers in each group. D, EPSCs at holding potentials of +40 mV and -70 mV in the presence of the NMDA receptor antagonist D-AP5 (50 μM) (top) and the calculated rectification index of EPSCs (bottom) in the three cell groups. E, EPSCs (top) and summarized results (bottom) before and during application of the GluA2-lacking AMPA receptor antagonist NASPM (100 μM) in the indicated cell groups. F, Evoked EPSC pairs (50 ms apart, top) and summarized data of paired-pulse ratios (bottom) in the three cell groups. * p < 0.05, ** p < 0.01.