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. Author manuscript; available in PMC: 2013 Jul 30.
Published in final edited form as: J Neurosci. 2013 Jan 23;33(4):1486–1497. doi: 10.1523/JNEUROSCI.4269-12.2013

Figure 2. Verification that Fluoro-Gold (FG) labels ChAT neurons in the DMV and that the recorded neurons are vagal premotor neurons.

Figure 2

A1–A3, Photomicrograph of DMV illustrating that ChAT-ir neurons (red) in DMV(A1) are also FG positive (blue) (A2). A3, Merged image of A1 and A2, double labeled neurons appear pink. Scale in A1, 100 µm, applies to A1–A3. B, Example of a slice reacted for immunohistochemistry to reveal the biocytin label (B1) showing the cell is also FG-ir (green, B2). B3, Merge of B1 and B2 showing the cell is within the DMV and also FG, thus projecting outside the brain. Scale in B1, 50 µm, applies to B1–B3. C1, C2, Illustrations of biocytin filled neurons recorded in slices and reconstructed showing the dendrites and axon projecting towards the periphery of the section. Scale in C2, 0.5 mm applies to C1–C2.