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. Author manuscript; available in PMC: 2013 Jul 30.
Published in final edited form as: J Neurosci. 2013 Jan 23;33(4):1486–1497. doi: 10.1523/JNEUROSCI.4269-12.2013

Figure 4. Axonal varicosities from C1 neurons in rat DMV contain PNMT and VGLUT2.

Figure 4

A, Photomicrograph showing that injection of AAV2-DIO-EYFP into the RVLM drives expression of EYFP in C1 (PNMT-ir) neurons in TH-Cre rats. EYFP immunoreactivity is in green and phenylethanolamine-N-methyl transferase (PNMT)-ir is in blue. Double labeled neurons appear aqua (arrows). Scale, 50 µm. B,Virtually all adrenergic varicosities (PNMT-ir, blue) within the DMV (B1, denoted by arrows or arrowheads) are also glutamatergic (VGLUT2-ir, red) (B2). The EYFP terminals (green) (B3, arrowheads) originating from C1 RVLM neurons are both adrenergic (red) and glutamatergic (green). B4, Merge of B1–B3. Triple labeled terminals (arrowheads) appear white. Double-label terminals (PNMT + VGLUT2) appear purple (arrows) when the colors are merged. C, Adrenergic terminals in DMV are also glutamatergic in naïve TH-Cre rats (no AAV2 injection). Adrenergic nerve terminals (PNMT-ir, red) located in DMV (C1) are also glutamatergic (VGLUT2-ir, green) (C2), arrows. These terminals appear yellow when the colors are merged in C3 (arrows). Note that practically all the PNMT-ir terminals are also VGLUT2-ir similar to the virus-injected rat shown in B. Scale in B1, 10 µm applies to B–C.