TRN neurons were recorded in voltage clamp with a Cs-based internal solution in the presence of R-CPP, picrotoxin, and CGP55845 to block NMDA, GABAA, and GABAB receptors, respectively. (A) Representative recording showing that low intensity stimuli trigger nEPSCs, blocked by DHβE (3 μM). Increases in stimulus intensity led to the recruitment of AMPAR EPSCs, blocked by NBQX (10 μM), while the nEPSC amplitude remained unchanged. (B) Summary plot showing distinct thresholds for AMPAR- and nEPSCs. n = 5.
Sun et al.