Table 1.

Primers and probes with their respective reporter dye and quencher. A standardized qPCR program was used for all three primer sets consisting of an initial incubation step at 95°C for 10 min, followed by 45 cycles of denaturation at 95°C for 10s and annealing/extension at 60°C for 30s.

Target bacteria		Sequence (5′-3′)
S. maltophilia	Forward primer	GATCCTGGCTCAGAGTGAACG
	Reverse primer	CCCACGACAGAGTAGATTCCG
	Probe	FAM-CACCCGTCCGCCACTCGCCAC-TAMRA
	Reference	Rios-Licea et al. (2010)
Streptomyces	Forward primer	GCCGATTGTGGTGAAGTGGA
	Reverse primer	GTACGGGCCGCCATGAAA
	Probe	FAM-ATCCTATGCTGTCGAGAAAAGCCTCTAGCG-TAMRA
	Reference	Rintala and Nevalainen (2006)
Mycobacterium	Forward primer	GATGCAACGCGAAGAACCTT
	Reverse primer	TGCACCACCTGCACACAGG
	Probe	FAM-CCTGGGTTTGACATGCACAGGACG-TAMRA
	Reference	Torvinen et al. (2010)