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. Author manuscript; available in PMC: 2013 Aug 13.
Published in final edited form as: J Neurosci. 2013 Feb 13;33(7):3178–3189. doi: 10.1523/JNEUROSCI.2428-12.2013

Figure 5. Hyper-isomerase activity and faster regeneration of 11cRAL in Fatp4−/− mice.

Figure 5

(A) Retinoid isomerase (RI) activities in WT and Fatp4−/− RPE cells. Synthesis of 11cROL by WT and Fatp4−/− RPE homogenates during the indicated incubation times was measured by HPLC. Asterisks indicate statistically significant differences (P < 0.05). Error bars show SD (n = 4). (B) Representative HPLC chromatograms of retinoids from WT and Fatp4−/− RPE homogenates assayed for 90 minutes in (A). The peaks corresponding to 11cROL are marked (arrow). (C) Immunoblot analysis showing similar expression levels of RPE65 in WT and Fatp4−/− RPE cells. RPE cells from RPE65-deficient rd12 mice were used as a negative control. (D) Relative contents of 11cRAL in the retinas from mice immediately after photo-bleaching (PB) of the visual pigment, or from mice kept in darkness for the indicated times after PB.