Skip to main content
. Author manuscript; available in PMC: 2013 Sep 6.
Published in final edited form as: J Neurosci. 2013 Mar 6;33(10):4405–4414. doi: 10.1523/JNEUROSCI.3940-12.2013

Figure 1. Low concentration copper and/or cimetidine treatment does not prevent cisplatin-induced hair cell death.

Figure 1

(a) Dose-response functions for treatment with cimetidine prior to 50 µM cisplatin for 24 hr. Increasing concentrations of cimetidine did not significantly protect against cisplatin-induced hair cell death. (b) Dose-response function of low concentration copper treatment alone or low concentration copper prior to 50 µM cisplatin for 24 hr. Copper concentrations of 0.01 – 0.25 µM alone did not cause significant hair cell toxicity. When used prior to and during cisplatin treatment there was no significant protection against cisplatin-induced hair cell death. (c) Dose-response functions demonstrating that combined treatment of 400 µM cimetidine and low concentration copper ranging from 0.05 –0.5 µM did not significantly protect against 50 µM cisplatin for 24 hr. (d) Dose-response functions for higher concentrations of cisplatin. Treatment with 0.25 µM copper, 400 µM cimetidine, or a combination of 0.25 µM copper and 400 µM cimetidine did not significantly protect against cisplatin treatment of 100, 250, or 500 µM for 6 hr. For all treatment groups (a–d), n = 9–12 fish; Error bars = SD; ***p< 0.001 by one-way ANOVA and Tukey-Kramer post-test.