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. Author manuscript; available in PMC: 2013 Sep 17.
Published in final edited form as: Results Immunol. 2012 Aug 27;2:166–172. doi: 10.1016/j.rinim.2012.08.002

Fig. 2. Proliferation of human mesangial cells stimulated with immune complexes formed in vitro using serum samples supplemented with different IgA1 myeloma proteins.

Fig. 2

Two different Gal-deficient IgA1 myeloma proteins, Mce and Gou (3 and 10 µg/ml final concentration), were added to cord-blood serum (CBS3), or serum from an IgAN patient or a healthy control (2% serum final concentration in the culture medium). After overnight incubation at 4 °C to allow formation of immune complexes, the samples were added to serum-starved human mesangial cells, and cellular proliferation was measured after 20-h incubation. Controls included stimulation with IgA1 proteins alone. Panel (a) shows proliferation with IgA1 proteins without allowing time for immune complex formation in serum samples (no complexes were formed) and panel (b) shows proliferation with IgA1-containing immune complexes. The changes in proliferation are shown as difference in thymidine uptake between a sample and a mock control (mock-treatment with no IgA1 added) (Δcpm). Mean and SD are shown (n = 6).