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. Author manuscript; available in PMC: 2008 Sep 19.
Published in final edited form as: J Neurosci. 2008 Mar 19;28(12):3150–3158. doi: 10.1523/JNEUROSCI.5753-07.2008

Figure 1. Fluorescent peptide with affinity for RIBEYE labels synaptic ribbons in live mouse bipolar cells.

Figure 1

A Bright-field image of an isolated mouse bipolar cell B. Confocal image of a different cell loaded with RIBEYE -binding peptide (Rpep). Bright spots (e.g., arrow) mark the positions of synaptic ribbons. C. Rpep-labeled bipolar cell terminal at higher magnification. Top panel shows a projection of a series of z-axis confocal optical sections through the depth of the terminal. Spots varied in size, suggesting that they might represent clusters with multiple ribbons. Bottom panel shows a single optical section of the same cell. D. A stereo pair showing a 3D reconstruction from optical sections through a bipolar cell synaptic terminal loaded with Rpep.