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. Author manuscript; available in PMC: 2013 Oct 24.
Published in final edited form as: J Neurosci. 2013 Apr 24;33(17):7384–7392. doi: 10.1523/JNEUROSCI.5839-12.2013

Figure 5.

Figure 5

Pharmacological profile of the zebrafish CACNA1Ab P/Q-type calcium channel expressed in HEK293T cells. (A) Representative recordings showing comparison of the block by 1 μM ω-conotoxin GVIA in 5 mM (filled symbols) and 10 mM (open symbols) Ba2+. The block is represented by the time-dependent reduction from pre-block peak current amplitude. Each time point was taken at 5 sec interval. The time of pressure application is indicated in each divalent concentration by the horizontal bars. The inset shows the Ba2+ current recorded pre-application (dark trace) and post-application (light trace) of ω-conotoxin GVIA. The test voltage steps corresponded to +10 mV from a holding potential of -80 mV. (B) The block by 1 μM ω-conotoxin MVIIC shown in the same manner as A. (C) The block by 2 μM ω-Agatoxin IVA is shown in the same manner as A. (D) Comparison of the current block by different toxins. Average percentage of block and n value (in parenthesis) for each condition are shown.