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Maintenance of ATII cell phenotype in cultured murine cells. ATII cells were cultured on 70% collagen/30% matrigel (CM) matrix for 5 or 7 days with or without keratinocyte growth factor (KGF) in the medium. Immunofluorescence assay was used to detect the expression of (A) ATII marker protein, LBP180, or (B) ATI marker protein, T1α. Cells cultured on fibronectin were used as a positive control for T1α expression.
