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. Author manuscript; available in PMC: 2013 Nov 1.
Published in final edited form as: J Neurosci. 2013 May 1;33(18):8068–8078. doi: 10.1523/JNEUROSCI.5351-12.2013

Figure 1. Elevation in cAMP cytosolic concentration increases prolactin (PRL) release from perifused pituitary lactotrophs.

Figure 1

Cultured pituitary lactotrophs were perifused with extracellular solutions containing different concentrations of forskolin, an adenyl cyclase activator, and dbcAMP (N6,2′-O-dibutyryl adenosine-3′,5′-cyclic monophosphate). Samples were collected every minute and analyzed for either cAMP or PRL. (a) Dose-dependent effect of forskolin on cAMP release in perifused lactotrophs. The grey part denotes the period of forskolin application. (b) Dose-dependent effect of forskolin on PRL release from perifused lactotrophs. The grey part denotes the period of forskolin application. (c) The effect of different concentrations of dbcAMP on the PRL release from perifused lactotrophs. The top x-axis shows the concentrations of applied dbcAMP. Shown are representative experiments from four independent experiments.