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. Author manuscript; available in PMC: 2008 Oct 29.
Published in final edited form as: J Bone Miner Res. 2008 Sep;23(9):1486–1496. doi: 10.1359/JBMR.080403

Figure 10.

Figure 10

Relative concentrations of VDR in the intestine following treatment of adult male zebrafish with vehicle, 1α, 25(OH)2D3 or lithocholic acid (LCA). * p<0.05. Adult zebrafish were administered 25 ng of 1α, 25(OH)2D3 in 5 μl of propylene glycol, 25 ng of lithocholic acid in 5 μl of propylene glycol, or vehicle (5 μl of propylene glycol) parenterally. Twenty two hours later the fish were killed and tissues harvested as described. Equal amounts of protein (60 μg) were separated by SDS-PAGE using 10% acrylamide gels. Following electrophoresis, proteins were transferred to PVDF membranes and the VDR was detected by Western blotting methods as described.