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. Author manuscript; available in PMC: 2013 Jul 11.
Published in final edited form as: J Vis Exp. 2012 Apr 15;(62):10.3791/3597 3597. doi: 10.3791/3597

Figure 4. Overall scheme of the nanoinjection/electrophysiology protocol.

Figure 4

A representative diagram of the overall scheme for the nanoinjection/electrophysiology protocol. Start by obtaining a baseline recording by stimulating the Giant Fibers (GFs) at 100 Hz with 10 trains of 10 stimuli each (only one train shown here). Before injection, begin the 1 Hz stimulations one second apart. During injection time (while injector is plugged in to the control box), you will observe significant background noise; however, do not discontinue the recordings. After injection (and injector is unplugged from control box), continue the 1 Hz stimulation for about 1 more minute. Finally, proceed to stress the GFs with 10 trains of 10 stimuli at 100 Hz and continue to test the function of the GF pathways with this paradigm every 5 minutes up to 15 minutes. Note: recordings were manipulated to create the overall scheme and do not represent a specific result obtained. Not to scale, not all traces are shown.