Table of specific reagents and equipment:

Name of the reagent	Company	Catalogue number	Comments (optional)
Recording glass electrodes: borosilicate glass capillaries	World Precision Instruments	1B100F-4	1.0mm OD, 0.58mm ID
Stimulator	Grass Instruments	Model S48
Amplifier	Getting Instruments Inc.	Model 5A
Data acquisition Software: Digidata	Molecular Devices®	Model 1440A
Data collection software: pCLAMP	Molecular Devices®	Version 10
Stereomicroscope with fiber optic microscope ring illuminator	AmScope	SM-4T Model HL250-AR
Dissecting scope for mounting	AmScope	SM-2TZ
Kite Manual Micromanipulator & Tilting Base	World Precision Instruments	Model # M3301 Kite: Model # KITE-M3-L
Drosophila melanogaster Wild 10E genotype (wild type strain)	Bloomington Stock center	Stock # 3892
Vertical pipette puller	David Kopf Instruments	Model 700c
Injection glass micropipettes: Borosilicate glass capillaries	World Precision Instruments	Catalogue # 4878	1.14mm OD, 0.5mm ID
Silicon oil	Fisher	Catalogue # S159-500
Beveler	Sutter Instrument Co.	K.T. Brown Type Model # BV-10
Nanoliter2000	World Precision Instruments	Catalogue # B203XVY
Blue food coloring	McCormick®	N/A	Ingredients: Water, Propylene Glycol, FD&C Blue 1, and 0.1% Propylparaben (preservative).
Methyllycaconitine citrate (MLA)	Tocris Bioscience	Catalogue # 1029
Plastic wax sticks	Hygenic Corporation (Akron Ohio USA)