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. Author manuscript; available in PMC: 2014 Sep 1.
Published in final edited form as: Antiviral Res. 2013 Jun 29;99(3):371–382. doi: 10.1016/j.antiviral.2013.06.014

Fig. 2. Dose-dependent inhibition of endosomal acidification caused by diphyllin.

Fig. 2

MDCK cells and A549 cells were incubated with bafilomycin A1 (0.2 μM) or various concentrations of diphyllin (0.078, 0.312, 1.25 μM) at 37°C for 20 min. Untreated cells (media only) were used as controls. Acridine orange dye (1 μg/ml) was added to each well and incubated for 10 min. (A) Acidic endosomes in cells were stained red by acridine orange and non-acidic endosomes were stained green. Fluorescence images were obtained on iCys Research Imaging Cytometer. Representative images are shown (magnification: 40×). (B) Fluorescence data was collected from diphyllin-treated wells and the green/red fluorescence ratio was presented. Data in the plot present the mean ± SD out of four replicates.