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. Author manuscript; available in PMC: 2014 Feb 5.
Published in final edited form as: Kidney Int. 2013 Aug 7;85(2):289–306. doi: 10.1038/ki.2013.290

Fig. 2. Hematoxylin and eosin (H and E) and picrosirius red staining of the LV-shRNA-VEGF-A transduced and Avastin treated vessels have increased lumen vessel area with decreased media and adventitia area and collagen expression.

Fig. 2

Fig. 2

Fig. 2

(A) First column is representative sections after hematoxylin and eosin (H and E) at the venous stenosis of the LV-shRNA-VEGF-A (LV) and scrambled-VEGF-A (C) transduced vessels or Avastin treated or IgG controls at day at day 14 showing increase in lumen vessel area. (A) Second column is representative polarized light microscopy of picrosirius red-stained venous stenosis showing decreased fibrosis (collagen fibers are bright yellow) of the LV-shRNA-VEGF-A (LV) and scrambled-VEGF-A (C) transduced vessels and Avastin treated vessels and controls. Qualitatively, there is a reduction in collagen staining by Sirius red by days 3–21. Bar is 200-μM. Pooled data for mean lumen vessel area LV and C groups and Avastintreated and control vessels are shown in B. There is a significant increase in the mean lumen vessel area in the LV transduced vessels when compared to C vessels for days 14–28 (all, P<0.001). By day 14, there was a significant increase in the mean lumen vessel area in the Avastin treated vessels when compared to IgG controls (P<0.001). Pooled data for mean media and adventitia area in LV and C groups and Avastin and control groups are shown in C. There is a significant decrease in the mean media and adventitia area in the LV transduced vessels when compared to C vessels for day 14 (P<0.01). By day 28, there is a significant reduction in the mean media and adventitia area in the Avastin treated vessels when compared to controls (P<0.001). D shows Kaplan-Meier estimates for LV transduced vessels (red) when compared to C vessels (blue). There is improved patency in the LV transduced vessels (red) when compared to C vessels (Log rank test: P<0.05). Each bar shows mean ± SEM of 4–6 animals per group (B–C). Two-way ANOVA followed by Student t-test with post hoc Bonferroni’s correction was performed. Significant difference from control value was indicated by **P < 0.001.