Table 1.

PCR primers used in current study

Gene	Sequence	Amplicon Length	Cycles
HIF-1α	5′ –agtgatgaaagaattact– 3′ (sense)	2759	35
	5′ –aataataccacttacaaca– 3′ (antisense)
VEGF-A	5′ –atgaagtgatcaagttcatgg– 3′ (sense)	360	35
	5′ –ggatcttggacaaacaaatgc– 3′ (antisense)
VEGFR-1	5′ –tttccatttgatactcttac– 3′ (sense)	310	35
	5′ –tcttagttgctttaccaggg– 3′ (antisense)
MMP-2	5′ – agatcttcttcttcaaggaccggtt– 3′ (sense)	225	35
	5′ – ggctggtcagtggcttggggta– 3′ (antisense)
MMP-9	5′ – gtttttgatgctattgctgagatcca– 3′ (sense)	208	35
	5′ – cccacatttgacgtccagagaagaa3′(antisense)
18S	5′ –agctaggaataatggaatag-3′ (sense)	150	19
	5′-aatcaagaacgaaagtcggag- 3′(antisense)