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. Author manuscript; available in PMC: 2013 Oct 19.
Published in final edited form as: Cancer Immunol Immunother. 2011 Oct 5;61(4):497–509. doi: 10.1007/s00262-011-1116-1

Fig. 1.

Fig. 1

Co-cultures in 3D model to assess the effects of Th1 cytokines and Her2Bi-armed ATC (aATC) on the development and regulatory activity of MDSC. PBMC were plated in matrigel at 1:10 (tumor cell/ PBMC). aATC were added after a week of tumor cells and PBMC co-culture. Following 3–5 days of additional co-culture, matrigel was digested and single-cell suspension was harvested for phenotyping or separation of CD33+ cells to evaluate their functional properties. Culture supernatants were assessed for cytokine levels