Table 2.
Type | Host | Origin | Protein | Number of monomers |
MW (KDa) | Cloning Plasmid | RE | Expression plasmid | Purification Strategy | Yield (mg/L) | References |
---|---|---|---|---|---|---|---|---|---|---|---|
Masp1 | 2 | 83 | pSLfa1180fa | Spe1/Nde1 | pBac[3xP3-DsRedaf] | N/A | [55] | ||||
Insects | B. mori | Nephila clavipes | 4 | 70 | pSL1180 | pFastBacHT-C | IMAC | 6mg/larva | [52] | ||
Masp1+Flag | multiple | 75–130 | pBSSKII+ and pSLfa1180fa | Sca1/Xma1/BspE1 | pBAC[3xP3-DsRedaf] | N/A | [53] | ||||
Nicotiana tobaccum Solaum tubercum | Masp1 | multi ple | 12.9–99.8 | pUC19 | NgoMIV/HindIII | pRTRA7/3 | (NH4)2SO4 10–50% saturation | 0.5–2 % total protein | [59] | ||
Plants | Nicotiana benthamiana | Nephila clavipes | Flag (intein) | 4; 10 | 47, 72, 100, 250 | pRTRA15 | splicing events | pCB301-Kan | IMAC | 1.8 mg/50 g leaft material 0.34%; 0.03% in leaves, | [62] |
Arabdopsis thaliana | Masp1 | 8, 16 | 64, 127 | pBSSK+ | BglII/BamH1 | Cong ‘ + Pha3’ | ammonium sulphate | 1.2%; 0.78% in seeds | [61] | ||
Glycine max | 1% in somatic embryos | ||||||||||
Trangenic mice | Nephila clavipes | MaSP1 | 6 | 31–66 | pGEM-5zf | BamHI/NcoI | pBC1 | centrifugation | 11.7 mg/L | [64] | |
Mammalians | COS- 1 c ells | Euprosthenops sp. | 25, 22 | pER1-14 | BamH1/EcoRV | pSecTag2/Hygro A | N/A | N/A | [49] | ||
Baby hamster kidney | Nephila clavipes | Masp1/ Masp2 | N/ A | 59, 106/ 59 | pBSSK+ | ApaI/ SapI | CMV promoter | ammonium sulfate | |||
Baby hamster kidney | Araneus diadematus | ADF3 | 63, 60, 110, 140 | pSecTag-C | MscI/ PvuII | 25–50 mg/L | [48] |