Table 2.

Summary of recombinantly expressed spider silks in insects, plants, and mammalians. Spider silk origin, number of monomers, molecular weight, cloning and expression plasmids as well as restriction enzymes and purification strategies used to produce recombinant silks are shown.

Type	Host	Origin	Protein	Number of monomers	MW (KDa)	Cloning Plasmid	RE	Expression plasmid	Purification Strategy	Yield (mg/L)	References
			Masp1	2	83	pSLfa1180fa	Spe1/Nde1	pBac[3xP3-DsRedaf]		N/A	[55]
Insects	B. mori	Nephila clavipes		4	70	pSL1180		pFastBacHT-C	IMAC	6mg/larva	[52]
			Masp1+Flag	multiple	75–130	pBSSKII+ and pSLfa1180fa	Sca1/Xma1/BspE1	pBAC[3xP3-DsRedaf]		N/A	[53]
	Nicotiana tobaccum Solaum tubercum		Masp1	multi ple	12.9–99.8	pUC19	NgoMIV/HindIII	pRTRA7/3	(NH4)2SO4 10–50% saturation	0.5–2 % total protein	[59]
Plants	Nicotiana benthamiana	Nephila clavipes	Flag (intein)	4; 10	47, 72, 100, 250	pRTRA15	splicing events	pCB301-Kan	IMAC	1.8 mg/50 g leaft material 0.34%; 0.03% in leaves,	[62]
	Arabdopsis thaliana		Masp1	8, 16	64, 127	pBSSK+	BglII/BamH1	Cong ‘ + Pha3’	ammonium sulphate	1.2%; 0.78% in seeds	[61]
	Glycine max									1% in somatic embryos
	Trangenic mice	Nephila clavipes	MaSP1	6	31–66	pGEM-5zf	BamHI/NcoI	pBC1	centrifugation	11.7 mg/L	[64]
Mammalians	COS- 1 c ells	Euprosthenops sp.			25, 22	pER1-14	BamH1/EcoRV	pSecTag2/Hygro A	N/A	N/A	[49]
	Baby hamster kidney	Nephila clavipes	Masp1/ Masp2	N/ A	59, 106/ 59	pBSSK+	ApaI/ SapI	CMV promoter	ammonium sulfate
	Baby hamster kidney	Araneus diadematus	ADF3		63, 60, 110, 140	pSecTag-C	MscI/ PvuII			25–50 mg/L	[48]