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. Author manuscript; available in PMC: 2015 Jan 1.
Published in final edited form as: J Innate Immun. 2013 Sep 25;6(2):227–239. doi: 10.1159/000354304

Fig. 8.

Fig. 8

Resistance to SP-A-mediated phagocytosis is regulated by RpoN. (a) Comparison of bacterial phagocytosis by RAW 264.7 macrophages between PAO1, ΔpilA and rpoN::ISphoA/hah in the presence or absence of 25 μg/ml hSP-A. All experiments were independently performed three times in triplicates. The mean ± standard deviation from one representative experiment is shown. *p < 0.05 when comparing PAO1 versus ΔpilA. #p < 0.05 when comparing PAO1 versus rpoN::ISphoA/hah. (b) Ligand blot analysis of nonpilus adhesins in PAO1, ΔpilA and rpoN::ISphoA/hah. (c) Image J analysis of the ligand blot. Protein expression was normalized to PAO1 as 100%. (d) Protein sequence of OprH. (e) Comparison of membrane permeabilization between PAO1, ΔpilA and rpoN::ISphoA/hah. The experiments were independently performed three times in triplicates. The mean ± standard deviation from one representative experiment is shown. *p < 0.05 when comparing PAO1 versus ΔpilA and rpoN::ISphoA/hah.