TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Description | Reference |
|---|---|---|
| P. aeruginosa strains | ||
| PAO1 | Wild-type | 3 |
| PAO1 ΔpilA | In frame deletion of the pilA gene | 3 |
| ΔpilA (comp) | Genetically-complemented ΔpilA mutant |
3 |
| PAO1 – GFP | PAO1 harboring pUCP19-gfp | This study |
| ΔpilA – GFP | ΔpilA harboring pUCP19-gfp | This study |
| ΔchpA | In frame deletion of the chpA gene | 3 |
| ΔpilG | In frame deletion of the pilG gene | 3 |
| ΔpilB | In frame deletion of the pilB gene | 3 |
| ΔpilH | In frame deletion of the pilH gene | 3 |
| ΔpilT CTX-PilU | In frame deletion of the pilT; pilU and 1 kb upstream sequence at attB site |
3 |
| ΔpilU | In frame deletion of the pilU gene | 3 |
| rpoN: :ISphoA/hah | Mutant harboring a ISphoA/hah transposon insertion into the rpoN gene |
University of Washington Genome Sciences |
| Plasmids | ||
| pUCP19-gfp | pUCP19 plasmic expressing a green fluorescence protein |
16 |