Fig. 2.

Protection against tumor growth by DNA versus cell vaccines in wild type mice. C57BL/6 or BALB/c mice were immunized with pE2TM and pGM-CSF or with SKOV3 cells twice, as described in Fig. 1 and mice were challenged s.c. with 2 × 10⁵ Her-2-transfected tumor cells at 2 weeks after the last vaccination. Tumor growth is expressed as % tumor-free mice. The number of mice per group is indicated on the graphs. a Immunized C57BL/6 mice were challenged with EL4/E2 tumors, b Immunized BALB/c mice were challenged with D2F2/E2 tumors. c SKOV3 immunized BALB/c mice were challenged with D2F2/E2 tumors. Some of the immunized mice were depleted of CD8⁺ T cells using mAb 2.43. * P < 0.05, ** P < 0.005 compared to vector–control mice by log-rank test