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The L1CD is not necessary for L1-mediated neurite outgrowth, but the L1CD regulates branching through the juxtamembrane region. L1KO neurons were transfected with WTL1 (A), L1-1147 (B), L1-4A(C), or L1-1151Y>A (D) and plated on an L1 substrate. After 48 hr, neurons were fixed and stained with a monoclonal anti-human L1 antibody (7B5). Scale bar, 100 µm.
