Table 3.
Summary of the muropeptide composition of mutant strains.
| Area - % of Each Muropeptidea | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
|
| |||||||||||
| Muropeptideb | WT (Avg ± SD)c | mviN | csd6tn | csd6 | csd6cmpl | csd6 OP | csd4c | csd4 OP | csd4 csd6 | csd1d | csd1 csd6 |
| Monomers (total) | 58.7 ± 1.7 | 57.3 | 57.4 | 57.5 | 57.8 | 57.6 | 60.0 | 56.7 | 57.0 | 53.3 | 54.0 |
|
| |||||||||||
| Di | 2.8 ± 0.4 | 1.7 | 0.6 | 0.5 | 2.8 | 4.4 | 0.0 | 4.0 | 0.2 | 2.3 | 0.0 |
|
| |||||||||||
| Tri | 4.0 ± 0.4 | 4.6 | 0.0 | 0.0 | 8.4 | 11.1 | 16.1 | 1.8 | 0.0 | 3.8 | 0.0 |
|
| |||||||||||
| Tetra | 10.0 ± 0.6 | 10.0 | 15.9 | 16.1 | 6.2 | 3.9 | 2.1 | 10.1 | 15.4 | 8.6 | 14.1 |
|
| |||||||||||
| Penta | 41.8 ± 1.1 | 41.0 | 40.9 | 41.0 | 40.4 | 38.2 | 41.8 | 40.8 | 41.4 | 38.6 | 39.9 |
|
| |||||||||||
| Dimers (total) | 41.3 ± 1.7 | 42.7 | 42.6 | 42.5 | 42.2 | 42.4 | 40.0 | 43.3 | 43.0 | 46.7 | 46.0 |
|
| |||||||||||
| TetraTri | 4.5 ± 0.3 | 4.5 | 0.0 | 0.0 | 8.5 | 11.7 | 13.2 | 3.3 | 0.0 | 4.0 | 0.0 |
|
| |||||||||||
| TetraTetra | 15.8 ± 0.3 | 15.8 | 20.7 | 21.1 | 11.9 | 9.8 | 7.5 | 16.7 | 21.5 | 16.8 | 20.3 |
|
| |||||||||||
| TetraPenta | 21.1 ± 1.3 | 22.4 | 21.9 | 21.3 | 21.8 | 20.9 | 19.3 | 23.3 | 21.5 | 26.0 | 25.7 |
|
| |||||||||||
| Chain ends (with anhydro-MurNAc) | 10.3 ± 0.6 | 10.3 | 10.5 | 10.3 | 10.2 | 10.9 | 9.0 | 10.7 | 10.4 | 10.5 | 10.6 |
|
| |||||||||||
| Avg. chain length | 9.7 ± 0.6 | 9.7 | 9.5 | 9.7 | 9.8 | 9.2 | 11.1 | 9.3 | 9.6 | 9.5 | 9.4 |
|
| |||||||||||
| % Peptides in cross-links | 41.3 ± 1.7 | 42.7 | 42.6 | 42.5 | 42.2 | 42.4 | 40.0 | 43.3 | 43.0 | 46.7 | 46.0 |
1
Percentages calculated as per (Glauner, 1988). Underlined values differ by more than 2 standard deviations from that of wild-type; underlined and bold values differ by more than 5 standard deviations from that of wild-type. Strains used: LSH100, TSH1, P1S1G1_5, TSH17, TSH31, TSH35, LSH28, LSH79, TSH27, LSH154, DBH11.
b
Nomenclature according to (Glauner, 1988).
c
Wild-type and csd4 mutant values as previously reported (Sycuro et al., 2012), the former calculated from 6 independent samples.
d
Similar to csd1 mutant values previously reported (Sycuro et al., 2010).