Fig. 6.

Olig1 or Olig2 knockdown mimics differentiation responses induced by inhibitor of DNA binding protein 2 or 4 (Id2 and Id4) overexpression in glioblastoma multiforme (GBM) neurospheres. In GBM neurosphere cells transfected with Olig1 or Olig2 siRNA for 72 h, differentiation markers GFAP, Tuj1 and GalC were examined by immunoblot (A), immnofluorescent staining (B) and quantitative RT-PCR (C). There was an increase in GFAP and Tuj1 expression and staining after Olig1 or Olig2 siRNA transfection in all three neurosphere lines. In contrast, GalC expression was decreased by Olig1 or Olig2 siRNA. Quantitative RT-PCR confirmed that Olig1 or Olig2 knockdown suppressed GalC expression (n = 3). *P < 0.05. Bar = 15 μm.