Expression levels of GM3NPhAc on FBL3 cells treated with ManNPhAc. After cells were incubated with 0, 0.02, 0.1, 0.5, and 2.0 mM of ManNPhAc for indicated time (24, 48, and 72 h), the cells were analyzed by ELISA using mAb 2H3 and alkaline phosphatase-linked goat anti-mouse IgM antibody as primary and secondary antibodies, respectively. GM3NPhAc levels were presented as the mean OD values at 450 nm obtained from triplicate experiments. * P < 0.05, #
P < 0.001 (vs control).