(A–F) PBMCs isolated from eighteen volunteers before and after (2 weeks, 3 months, and 1 year) vaccination were stimulated in vitro with sonicated M. tuberculosis (A,D), heat-killed C. albicans yeast, (B,E), and S. aureus (C,F). IL-17 (A–C) and IL-22 (D–F) production was assessed by ELISA in the supernatants. *p<0.05, **p<0.01, ***p<0.005.